品牌 |
Leading Biology |
貨號 |
APR15287G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
|
產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CCR7 antibody (N-term).
|
分子量 |
42874 Da
|
細胞定位 |
Antigen Cellular Localization:
Cell membrane; Multi-pass membrane protein.
|
宿主 |
Rabbit
|
種屬反應(yīng)性 |
Human, Mouse
|
免疫原 |
11-37 aa
|
靶點 |
This CCR7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-37 amino acids from the N-terminal region of human CCR7.
|
亞型 |
Rabbit Ig
|
通用名 |
CMKBR7, EBI1, EVI1
|
基因ID |
|
UniProt ID |
|
功能 |
Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
|
總結(jié) |
CCR7 is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor.
|
形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
|
儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
|
應(yīng)用 |
WB, IHC-P, IF, FC, E
|
稀釋方法 |
WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
FC~~1:10~50
|
別名 |
C-C chemokine receptor type 7, C-C CKR-7, CC-CKR-7, CCR-7, BLR2, CDw197, Epstein-Barr virus-induced G-protein coupled receptor 1, EBI1, EBV-induced G-protein coupled receptor 1, MIP-3 beta receptor, CD197, CCR7, CMKBR7, EBI1, EVI1
|
圖像 |
All lanes : Anti-CCR7 Antibody (N-term) at 1:500-1:1000 dilution
Lane 1: MDA-MB-231 whole cell lysate
Lane 2: MOLT-4 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 43 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-CCR7 Antibody (Nterm) at 1:500 dilution + MOLT-4 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 43 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
CCR7 Antibody (N-term) (Cat. APR15287G) western blot analysis in 293(lane 1),Ramos(lane 2),MDA-MB231(lane 3) cell line and mouse spleen tissue(lane 4) lysates (35ug/lane).This demonstrates the CCR7 antibody detected the CCR7 protein (arrow).
|
說明書 |
|
數(shù)量 |
|