ANKFY1 Antibody (C-term)

APR14928G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR14928G		100 μl / ￥4950

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ANKFY1 Antibody (C-term)

品牌	Leading Biology	貨號(hào)	APR14928G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ANKFY1 antibody (C-term).
分子量	128399 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm. Endosome membrane; Peripheral membrane protein. Early endosome. Note=Also associated with endosomal membranes. Localizes to macropinosomes
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	436-465 aa
靶點(diǎn)	This ANKFY1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 436-465 amino acids from the C-terminal region of human ANKFY1.
亞型	Rabbit Ig
通用名	ANKHZN, KIAA1255
基因ID	51479
UniProt ID	Q9P2R3
功能	Proposed effector of Rab5. Binds to phosphatidylinositol 3-phosphate (PI(3)P). Involved in homotypic early endosome fusion and to a lesser extend in heterotypic fusion of chlathrin-coated vesicles with early endosomes. Involved in macropinocytosis; the function is dependent on Rab5-GTP. Required for correct endosomal localization. Involved in the internalization and trafficking of activated tyrosine kinase receptors such as PDGFRB. Regulates the sucellular localization of the retromer complex in a EHD1- dependent manner. Involved in endosome-to-Golgi transport and biosynthetic transport to late endosomes and lysosomes indicative for a regulation of retromer complex-mediated retrograde transport.
總結(jié)	ANKFY1 encodes a cytoplasmic protein that contains a coiled-coil structure and a BTB/POZ domain at its N-terminus, ankyrin repeats in the middle portion, and a FYVE-finger motif at its C-terminus. This protein belongs to a subgroup of double zinc finger proteins which may be involved in vesicle or protein transport.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, FC, E
稀釋方法	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50
別名	Rabankyrin-5, Rank-5, Ankyrin repeat and FYVE domain-containing protein 1, Ankyrin repeats hooked to a zinc finger motif, ANKFY1, ANKHZN, KIAA1255
圖像	ANKFY1 Antibody (C-term) (Cat. APR14928G) western blot analysis in MDA-MB435,CEM,MCF-7,Jurkat,Hela cell line lysates (35ug/lane).This demonstrates the ANKFY1 antibody detected the ANKFY1 protein (arrow). ANKFY1 Antibody (C-term) (Cat. APR14928G) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ANKFY1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. ANKFY1 Antibody (C-term) (Cat. APR14928G) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
說(shuō)明書(shū)	Product Datasheet Protocol
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