Phospho-STAT5a(Y694) Antibody

APR07156G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR07156G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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Phospho-STAT5a(Y694) Antibody

品牌	Leading Biology	貨號(hào)	APR07156G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Phospho-STAT5a(Y694) antibody.
分子量	90647 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation
宿主	Rabbit
種屬反應(yīng)性	Human
靶點(diǎn)	This STAT5a Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y694 of human STAT5a.
亞型	Rabbit Ig
通用名	STAT5
基因ID	6776
UniProt ID	P42229
功能	Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
總結(jié)	STAT5a is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this protein is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IF, IHC-P, E
稀釋方法	WB~~1:1000 IF~~1:200 IHC-P~~1:50~100
別名	Signal transducer and activator of transcription 5A, STAT5A, STAT5
圖像	Western blot analysis of anti-Phospho-STAT5 Pab(Cat. APR07156G) in HepG2 cell line lysate (35ug/lane). Phospho-STAT5(arrow) was detected using the purified Pab. Fluorescent confocal image of an SY5Y cell stained with APR07156G Phospho-STAT5a antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with APR07156G Phospho-STAT5a primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 3 min)(not shown). Phospho-STAT5a immunoreactivity is localized exclusively to the nucleus. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
說(shuō)明書(shū)	Product Datasheet Protocol
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