品牌 |
Leading Biology |
貨號 |
APR10522G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality TPI1 antibody (N-term).
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分子量 |
30791 Da
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Mouse
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免疫原 |
68-96 aa
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靶點 |
This TPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-96 amino acids from the N-terminal region of human TPI1.
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亞型 |
Rabbit Ig
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通用名 |
TPI
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基因ID |
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UniProt ID |
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總結(jié) |
TPI1 is an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
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別名 |
Triosephosphate isomerase, TIM, Triose-phosphate isomerase, TPI1, TPI
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圖像 |
All lanes : Anti-TPI1 Antibody (N-term) at 1:1000 dilution
Lane 1: A431 whole cell lysate
Lane 2: HepG2 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 31 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of TPI1 Antibody (N-term) (Cat. APR10522G) in Y79(lane 1),CEM(lane 2) cell line and mouse brain tissue(lane 3) lysates (35ug/lane). TPI1 (arrow) was detected using the purified Pab.(2ug/ml)
Western blot analysis of TPI1 (arrow) using rabbit polyclonal TPI1 Antibody (N-term) (Cat. APR10522G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TPI1 gene.
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說明書 |
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數(shù)量 |
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