SMPD2 Antibody (N-term)

APR13414G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR13414G		100 μl / ￥4950

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SMPD2 Antibody (N-term)

品牌	Leading Biology	貨號(hào)	APR13414G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SMPD2 antibody (N-term).
分子量	47646 Da
細(xì)胞定位	Antigen Cellular Localization: Membrane; Multi-pass membrane protein.
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse
免疫原	9-35 aa
靶點(diǎn)	This SMPD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-35 amino acids from the N-terminal region of human SMPD2.
亞型	Rabbit Ig
基因ID	6610
UniProt ID	O60906
功能	Converts sphingomyelin to ceramide. Hydrolyze 1-acyl-2- lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso- sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF.
總結(jié)	SMPD2 converts sphingomyelin to ceramide and hydrolyzes 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, FC, E
稀釋方法	WB~~1:1000 FC~~1:10~50
別名	Sphingomyelin phosphodiesterase 2, Lyso-platelet-activating factor-phospholipase C, Lyso-PAF-PLC, Neutral sphingomyelinase, N-SMase, nSMase, SMPD2
圖像	All lanes : Anti-SMPD2 Antibody (N-term) at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of SMPD2 Antibody (N-term) (Cat. APR13414G) in mouse lung tissue lysates (35ug/lane). SMPD2 (arrow) was detected using the purified Pab. SMPD2 Antibody (N-term) (Cat. APR13414G) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
說明書	Product Datasheet Protocol
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