品牌 |
Leading Biology | 貨號(hào) |
APR08839G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality NSE antibody (Y236).
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分子量 |
47269 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
213-239 aa
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靶點(diǎn) |
This NSE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 213-239 amino acids from human NSE.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
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總結(jié) |
NSE is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
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別名 |
Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2
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圖像 |
Western blot analysis of anti-NSE Antibody (Y236) (Cat. APR08839G) in Y79 cell line lysates (35ug/lane). NSE (arrow) was detected using the purified Pab. NSE Antibody (Y236) (Cat. APR08839G)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NSE Antibody (Y236) for immunohistochemistry. Clinical relevance has not been evaluated. |
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說(shuō)明書 |
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數(shù)量 |
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