品牌 |
Leading Biology |
貨號 |
AMM08727G |
產品分類 |
Polyclonal Antibodies |
研究領域 |
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產品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality (Mouse) Pou5f1 antibody (Center).
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分子量 |
38216 Da
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細胞定位 |
Antigen Cellular Localization:
Cytoplasm. Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000255|PROSITE- ProRule:PRU00530, ECO:0000269|PubMed:17496161, ECO:0000269|PubMed:17525163}. Note=Expressed in a diffuse and slightly punctuate pattern
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宿主 |
Rabbit
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種屬反應性 |
Human, Mouse
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靶點 |
This Mouse Pou5f1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 214-247 amino acids from the Central region of Mouse Pou5f1.
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亞型 |
Rabbit Ig
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通用名 |
Oct-3, Oct-4, Otf-3, Otf3
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基因ID |
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UniProt ID |
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功能 |
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
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總結 |
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應用 |
WB, E
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稀釋方法 |
WB~~1:4000
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別名 |
POU domain, class 5, transcription factor 1, NF-A3, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3, Pou5f1, Oct-3, Oct-4, Otf-3, Otf3
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圖像 |
All lanes : Anti-Pou5f1 Antibody (Center) at 1:4000 dilution
Lane 1: F9 whole cell lysates
Lane 2: NCCIT whole cell lysates
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 38 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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說明書 |
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數量 |
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