品牌 |
Leading Biology |
貨號(hào) |
APC00103G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality FGF2 antibody.
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分子量 |
30770 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Secreted. Nucleus. Note=Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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靶點(diǎn) |
This FGF2 antibody is generated from a rabbit immunized with a recombinant protein.
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亞型 |
Rabbit Ig
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通用名 |
FGFB
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基因ID |
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UniProt ID |
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功能 |
Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.
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總結(jié) |
Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, E
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稀釋方法 |
WB~~1:2000
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別名 |
Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, bFGF, Heparin-binding growth factor 2, HBGF-2, FGF2, FGFB
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圖像 |
Anti-FGF2 Antibodyat 1:2000 dilution + A549 whole cell lysates
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 31 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-FGF2 Antibody at 1:2000 dilution
Lane 1: A549 whole cell lysates
Lane 2: mouse heart lysates
Lane 3: rat lung lysates
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 31 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-FGF2 Antibodyat 1:2000 dilution + K562 whole cell lysates
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 31 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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說明書 |
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數(shù)量 |
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