品牌 |
Leading Biology | 貨號(hào) |
APG03321G |
產(chǎn)品分類(lèi) |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality GFAP antibody (N-term).
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分子量 |
49880 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm. Note=Associated with intermediate filaments
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
10-40 aa
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靶點(diǎn) |
This GFAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-40 amino acids from the N-terminal region of human GFAP.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
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總結(jié) |
GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, E
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稀釋方法 |
WB~~1:1000
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別名 |
Glial fibrillary acidic protein, GFAP, GFAP
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圖像 |
All lanes : Anti-GFAP Antibody (M1) at 1:8000 dilution Lane 1: human brain lysate Lane 2: human cerebellum lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of GFAP (arrow) using GFAP Antibody (N-term) (Cat. APG03321G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GFAP gene (Lane 2) (Origene Technologies). |
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說(shuō)明書(shū) |
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數(shù)量 |
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