品牌 |
Leading Biology |
貨號(hào) |
APR10193G |
產(chǎn)品分類(lèi) |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality SNAP25 antibody (Center).
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分子量 |
23315 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm, perinuclear region. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome Note=Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region (By similarity).
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
36-63 aa
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靶點(diǎn) |
This SNAP25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-63 amino acids from the Central region of human SNAP25.
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亞型 |
Rabbit Ig
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通用名 |
SNAP
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基因ID |
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UniProt ID |
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功能 |
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
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總結(jié) |
Synaptic vesicle membrane docking and fusion is mediatedby SNAREs (soluble N-ethylmaleimide-sensitive factor attachmentprotein receptors) located on the vesicle membrane (v-SNAREs) andthe target membrane (t-SNAREs). The assembled v-SNARE/t-SNAREcomplex consists of a bundle of four helices, one of which issupplied by v-SNARE and the other three by t-SNARE. For t-SNAREs onthe plasma membrane, the protein syntaxin supplies one helix andthe protein encoded by this gene contributes the other two.Therefore, this gene product is a presynaptic plasma membraneprotein involved in the regulation of neurotransmitter release. Twoalternative transcript variants encoding different protein isoformshave been described for this gene.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, E
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稀釋方法 |
WB~~1:1000
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別名 |
Synaptosomal-associated protein 25, SNAP-25, Super protein, SUP, Synaptosomal-associated 25 kDa protein, SNAP25, SNAP
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圖像 |
Anti-SNAP25 Antibody (Center) at 1:1000 dilution + SH-SY5Y whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 23 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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說(shuō)明書(shū) |
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數(shù)量 |
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