品牌 |
Leading Biology | 貨號(hào) |
APC00083G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Mouse Eef2k antibody (N-term).
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分子量 |
81739 Da
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Mouse
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免疫原 |
178-205 aa
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靶點(diǎn) |
This Mouse Eef2k antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-205 amino acids from the N-terminal region of mouse Eef2k.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
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總結(jié) |
Phosphorylates eukaryotic elongation factor-2. Binds calmodulin (By similarity).
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, E
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稀釋方法 |
WB~~1:1000
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別名 |
Eukaryotic elongation factor 2 kinase, eEF-2 kinase, eEF-2K, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, Eef2k
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圖像 |
Anti-Mouse Eef2k Antibody (N-term) at 1:1000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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說(shuō)明書 |
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數(shù)量 |
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