HMGN1 Antibody (N-term)

APR12388G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR12388G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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HMGN1 Antibody (N-term)

品牌	Leading Biology	貨號(hào)	APR12388G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality HMGN1 antibody (N-term).
分子量	10659 Da
細(xì)胞定位	Antigen Cellular Localization: Nucleus. Cytoplasm. Note=Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	1-30 aa
靶點(diǎn)	This HMGN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HMGN1.
亞型	Rabbit Ig
通用名	HMG14
基因ID	3150
UniProt ID	P05114
功能	Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 (By similarity).
總結(jié)	Chromosomal protein HMG14 and its close analog HMG17 (MIM163910) bind to the inner side of the nucleosomal DNA, potentiallyaltering the interaction between the DNA and the histone octamer.The 2 proteins may be involved in the process that maintainstranscribable genes in a unique chromatin conformation. Theirubiquitous distribution and relative abundance, as well as the highevolutionary conservation of the DNA-binding domain of the HMG14family of proteins, suggest that they may be involved in animportant cellular function.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:10~50
別名	Non-histone chromosomal protein HMG-14, High mobility group nucleosome-binding domain-containing protein 1, HMGN1, HMG14
圖像	HMGN1 Antibody (N-term) (Cat. APR12388G) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the HMGN1 antibody detected the HMGN1 protein (arrow). Western blot analysis of HMGN1 (arrow) using rabbit polyclonal HMGN1 Antibody (N-term) (Cat. APR12388G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HMGN1 gene. HMGN1 Antibody (N-term) (APR12388G)immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HMGN1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
說明書	Product Datasheet Protocol
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