CD3Z Antibody (N-term)

APR15350G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR15350G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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CD3Z Antibody (N-term)

品牌	Leading Biology	貨號(hào)	APR15350G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CD3Z antibody (N-term).
分子量	18696 Da
細(xì)胞定位	Antigen Cellular Localization: Membrane; Single-pass type I membrane protein
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	2-31 aa
靶點(diǎn)	This CD3Z antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-31 amino acids from the N-terminal region of human CD3Z.
亞型	Rabbit Ig
通用名	CD3Z, T3Z, TCRZ
基因ID	919
UniProt ID	P20963
功能	Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
總結(jié)	T-cell receptor zeta, together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, FC, E
稀釋方法	WB~~1:1000 FC~~1:10~50
別名	T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD247, CD247, CD3Z, T3Z, TCRZ
圖像	All lanes : Anti-CD3Z Antibody (N-term) at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: MOLT-4 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of anti-CD3Z Antibody (N-term) (RB13817) in Ramos cell line lysates (35ug/lane). CD3Z(arrow) was detected using the purified Pab. Flow cytometric analysis of Ramos cells using CD3Z Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
說明書	Product Datasheet Protocol
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