品牌 |
Leading Biology | 貨號 |
APR15712G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality DDX4 antibody (C-term).
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分子量 |
79308 Da
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細胞定位 |
Antigen Cellular Localization:
Cytoplasm. Cytoplasm, perinuclear region
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
554-583 aa
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靶點 |
This DDX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 554-583 amino acids from the C-terminal region of human DDX4.
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亞型 |
Rabbit Ig
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通用名 |
VASA
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基因ID |
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UniProt ID |
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功能 |
May play a role in germ cell development. May play a role in sperm motility.
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總結(jié) |
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:10~50
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別名 |
Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Vasa homolog, DDX4, VASA
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圖像 |
Anti-DDX4 Antibody (C-term) at 1:1000 dilution + human ovary lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 79 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Formalin-fixed and paraffin-embedded human testis tissue reacted with DDX4 antibody (C-term) (Cat. APR15712G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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說明書 |
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數(shù)量 |
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