品牌 |
Leading Biology | 貨號(hào) |
AMM08128G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality TCF3 antibody (C-term).
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分子量 |
67600 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Nucleus.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Mouse
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免疫原 |
506-534 aa
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靶點(diǎn) |
This TCF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 506-534 amino acids from the C-terminal region of human TCF3.
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亞型 |
Rabbit Ig
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通用名 |
BHLHB21, E2A, ITF1
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基因ID |
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UniProt ID |
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功能 |
Transcriptional regulator. Involved in the initiation of neuronal differentiation. Heterodimers between TCF3 and tissue- specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E- box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region.
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總結(jié) |
The TCF3 gene, also called E2A, encodes 2 basichelix-loop-helix (bHLH) transcription factors, E12 and E47, throughalternative splicing. E12 and E47 are involved in regulation ofimmunoglobulin gene expression (Bain et al., 1994 [PubMed8001125]).
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, FC, IHC-P, E
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稀釋方法 |
WB~~1:1000
FC~~1:10~50
IHC-P~~1:10~50
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別名 |
Transcription factor E2-alpha, Class B basic helix-loop-helix protein 21, bHLHb21, Immunoglobulin enhancer-binding factor E12/E47, Immunoglobulin transcription factor 1, Kappa-E2-binding factor, Transcription factor 3, TCF-3, Transcription factor ITF-1, TCF3, BHLHB21, E2A, ITF1
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圖像 |
All lanes : Anti-TCF3 Antibody (C-term) at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Ramos whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST. TCF3 Antibody (C-term) (Cat. AMM08128G) western blot analysis in human uterine tumor tissue lysates (35ug/lane).This demonstrates the TCF3 antibody detected the TCF3 protein (arrow). TCF3 Antibody (C-term) (Cat. AMM08128G) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the TCF3 antibody detected the TCF3 protein (arrow). |
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說明書 |
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數(shù)量 |
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