Beclin 1 Antibody

APG02270G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APG02270G		100 μl / ￥4950

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Beclin 1 Antibody

品牌	Leading Biology	貨號	APG02270G
產(chǎn)品分類	Polyclonal Antibodies	研究領域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Beclin 1 antibody.
分子量	51896 Da
細胞定位	Antigen Cellular Localization: Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity)
宿主	Rabbit
種屬反應性	Human
免疫原	100-123 aa
靶點	This Beclin 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-123 amino acids from human Beclin 1.
亞型	Rabbit Ig
通用名	GT197
基因ID	8678
UniProt ID	Q14457
功能	Plays a central role in autophagy (PubMed:23184933). Required for the abcission step in cytokinesis (PubMed:20208530). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense.
總結(jié)	Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).[supplied by OMIM].
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應用	WB, IF, E
稀釋方法	WB~~1:2000 IF~~1:100
別名	Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, BECN1, GT197
圖像	Anti-Beclin 1 Antibody at 1:2000 dilution + Raji whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Fluorescent image of U251 cells stained with Beclin 1 antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with APG02270G Beclin 1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
說明書	Product Datasheet Protocol
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