品牌 |
Leading Biology | 貨號(hào) |
APR16040G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality FSTL3 antibody (C-term).
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分子量 |
27663 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Isoform 1: Secreted.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
229-258 aa
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靶點(diǎn) |
This FSTL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-258 amino acids from the C-terminal region of human FSTL3.
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亞型 |
Rabbit Ig
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通用名 |
FLRG
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基因ID |
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UniProt ID |
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功能 |
Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiationc. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 or the nuclear form is probably involved in transcriptional regulation via interaction with MLLT10.
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總結(jié) |
Follistatin-like 3 is a secreted glycoprotein of thefollistatin-module-protein family. It may have a role inleukemogenesis.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
IHC-P, WB, E
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稀釋方法 |
IHC-P~~1:25
WB~~1:1000
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別名 |
Follistatin-related protein 3, Follistatin-like protein 3, Follistatin-related gene protein, FSTL3, FLRG
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圖像 |
AP12300B staining FSTL3 in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. Anti-FSTL3 Antibody (C-term) at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Anti-FSTL3 Antibody (C-term) at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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說(shuō)明書(shū) |
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數(shù)量 |
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