品牌 |
Leading Biology | 貨號 |
APR12224G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality GPR45 antibody (Center).
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分子量 |
41967 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cell membrane; Multi-pass membrane protein.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
121-149 aa
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靶點(diǎn) |
This GPR45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-149 amino acids from the Central region of human GPR45.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Orphan receptor. May play a role in brain function.
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總結(jié) |
This intronless gene encodes a member of the G protein-coupled receptor (GPCR) family. Members of this protein family contain seven putative transmembrane domains and may mediate signaling processes to the interior of the cell via activation of heterotrimeric G proteins. This protein may function in the central nervous system.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:10~50
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別名 |
Probable G-protein coupled receptor 45, PSP24-1, PSP24-alpha, GPR45
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圖像 |
Anti-GPR45 Antibody (Center) at 1:2000 dilution + human cerebellum lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST. All lanes : Anti-GPR45 Antibody (Center) at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST. GPR45 Antibody (Center) (Cat. APR12224G) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the GPR45 antibody detected the GPR45 protein (arrow). |
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說明書 |
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數(shù)量 |
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