RASGRP2 Antibody (N-term)

AMM07541G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
AMM07541G		100 μl / ￥4950

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RASGRP2 Antibody (N-term)

品牌	Leading Biology	貨號(hào)	AMM07541G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality RASGRP2 antibody (N-term).
分子量	69248 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Cell junction, synapse, synaptosome. Cell projection, ruffle membrane; Peripheral membrane protein. Note=Found both in the cytosol and associated with membranes. Isoform 2 mainly localizes to the cell membrane. Enriched at juxtamembrane areas and membrane ruffles through association with F-actin. Localizes to the cell bodies and axons of striatal neurons (By similarity)
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	7-34 aa
靶點(diǎn)	This RASGRP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-34 amino acids from the N-terminal region of human RASGRP2.
亞型	Rabbit Ig
通用名	CDC25L, MCG7
基因ID	10235
UniProt ID	Q7LDG7
功能	Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.
總結(jié)	The protein encoded by this gene is a brain-enrichednucleotide exchanged factor that contains an N-terminal GEF domain,2 tandem repeats of EF-hand calcium-binding motifs, and aC-terminal diacylglycerol/phorbol ester-binding domain. Thisprotein can activate small GTPases, including RAS and RAP1/RAS3.The nucleotide exchange activity of this protein can be stimulatedby calcium and diacylglycerol. Three alternatively splicedtranscript variants encoding the same protein have been found forthis gene.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, IF, E
稀釋方法	WB~~1:1000 IHC-P~~1:10~50 IF~~1:10~50
別名	RAS guanyl-releasing protein 2, Calcium and DAG-regulated guanine nucleotide exchange factor I, CalDAG-GEFI, Cdc25-like protein, hCDC25L, F25B33 kinase-like protein, RASGRP2, CDC25L, MCG7
圖像	Anti-RASGRP2 Antibody (N-term) at 1:1000 dilution + human spleen lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 69 kDa Blocking/Dilution buffer: 5% NFDM/TBST. RASGRP2 Antibody (N-term) (Cat. AMM07541G) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RASGRP2 antibody detected the RASGRP2 protein (arrow). RASGRP2 Antibody (N-term) (Cat. AMM07541G)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RASGRP2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
說(shuō)明書	Product Datasheet Protocol
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