品牌 |
Leading Biology | 貨號(hào) |
APR10984G |
產(chǎn)品分類(lèi) |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality AP1M1 antibody (Center).
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分子量 |
48587 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
199-227 aa
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靶點(diǎn) |
This AP1M1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 199-227 amino acids from the Central region of human AP1M1.
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亞型 |
Rabbit Ig
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通用名 |
CLTNM
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基因ID |
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UniProt ID |
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功能 |
Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
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總結(jié) |
The protein encoded by this gene is the medium chain ofthe trans-Golgi network clathrin-associated protein complex AP-1.The other components of this complex are beta-prime-adaptin,gamma-adaptin, and the small chain AP1S1. This complex is locatedat the Golgi vesicle and links clathrin to receptors in coatedvesicles. These vesicles are involved in endocytosis and Golgiprocessing. Alternatively spliced transcript variants encodingdistinct protein isoforms have been found for this gene. [providedby RefSeq].
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, IF, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
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別名 |
AP-1 complex subunit mu-1, AP-mu chain family member mu1A, Adaptor protein complex AP-1 subunit mu-1, Adaptor-related protein complex 1 subunit mu-1, Clathrin assembly protein complex 1 mu-1 medium chain 1, Clathrin coat assembly protein AP47, Clathrin coat-associated protein AP47, Golgi adaptor HA1/AP1 adaptin mu-1 subunit, Mu-adaptin 1, Mu1A-adaptin, AP1M1, CLTNM
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圖像 |
Western blot analysis of lysates from A375 cell line and rat testis tissue lysate(from left to right), using AP1M1 Antibody (Center)( APR10984G). APR10984G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. AP1M1 Antibody (Center) (Cat. APR10984G)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AP1M1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. Confocal immunofluorescent analysis of AP1M1 Antibody (Center)( APR10984G) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
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說(shuō)明書(shū) |
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數(shù)量 |
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