品牌 |
Leading Biology | 貨號(hào) |
APR12782G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality PCDHGA8 antibody (C-term).
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分子量 |
101480 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cell membrane; Single-pass type I membrane protein
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
781-808 aa
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靶點(diǎn) |
This PCDHGA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 781-808 amino acids from the C-terminal region of human PCDHGA8.
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亞型 |
Rabbit Ig
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通用名 |
KIAA0327
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基因ID |
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UniProt ID |
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功能 |
Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.
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總結(jié) |
This gene is a member of the protocadherin gamma genecluster, one of three related clusters tandemly linked onchromosome five. These gene clusters have an immunoglobulin-likeorganization, suggesting that a novel mechanism may be involved intheir regulation and expression. The gamma gene cluster includes 22genes divided into 3 subfamilies. Subfamily A contains 12 genes,subfamily B contains 7 genes and 2 pseudogenes, and the moredistantly related subfamily C contains 3 genes. The tandem array of22 large, variable region exons are followed by a constant region,containing 3 exons shared by all genes in the cluster. Eachvariable region exon encodes the extracellular region, whichincludes 6 cadherin ectodomains and a transmembrane region. Theconstant region exons encode the common cytoplasmic region. Theseneural cadherin-like cell adhesion proteins most likely play acritical role in the establishment and function of specificcell-cell connections in the brain. Alternative splicing has beendescribed for the gamma cluster genes.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, IF, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
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別名 |
Protocadherin gamma-A8, PCDH-gamma-A8, PCDHGA8, KIAA0327
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圖像 |
PCDHGA8 Antibody (C-term) (Cat. APR12782G) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PCDHGA8 antibody detected the PCDHGA8 protein (arrow). PCDHGA8 Antibody (C-term) (Cat. APR12782G)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDHGA8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. Confocal immunofluorescent analysis of PCDHGA8 Antibody (C-term)( APR12782G) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
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說(shuō)明書(shū) |
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數(shù)量 |
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