品牌 |
Leading Biology | 貨號(hào) |
APR08447G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality MIA40 antibody (C-term).
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分子量 |
15996 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Mitochondrion intermembrane space
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
101-129 aa
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靶點(diǎn) |
This MIA40 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101-129 amino acids from the C-terminal region of human MIA40.
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亞型 |
Rabbit Ig
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通用名 |
MIA40
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基因ID |
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UniProt ID |
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功能 |
Functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17. Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS. Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay system.
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總結(jié) |
CHCHD4, a component of human mitochondria, belongs to aprotein family whose members share 6 highly conserved cysteineresidues constituting a -CXC-CX(9)C-CX(9)C- motif in the C terminus(Hofmann et al., 2005 [PubMed 16185709]).
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:10~50
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別名 |
Mitochondrial intermembrane space import and assembly protein 40, Coiled-coil-helix-coiled-coil-helix domain-containing protein 4, CHCHD4, MIA40
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圖像 |
MIA40 Antibody (C-term) (Cat. APR08447G) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the MIA40 antibody detected the MIA40 protein (arrow). MIA40 Antibody (C-term) (Cat. APR08447G)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MIA40 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
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說明書 |
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數(shù)量 |
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