品牌 |
Leading Biology | 貨號(hào) |
APR10131G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality SLC5A12 antibody (C-term).
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分子量 |
67647 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Apical cell membrane; Multi-pass membrane protein. Note=Detected at the brush border membrane of the kidney. Colocalizes with viementin in Mueller cells (By similarity).
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
576-605 aa
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靶點(diǎn) |
This SLC5A12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 576-605 amino acids from the C-terminal region of human SLC5A12.
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亞型 |
Rabbit Ig
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通用名 |
SMCT2
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基因ID |
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UniProt ID |
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功能 |
Acts as an electroneutral and low-affinity sodium (Na(+))-dependent sodium-coupled solute transporter. Catalyzes the transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, nicotinate, propionate, butyrate and beta-D- hydroxybutyrate. May be responsible for the first step of reabsorption of monocarboxylates from the lumen of the proximal tubule of the kidney and the small intestine. May play also a role in monocarboxylates transport in the retina (By similarity). Mediates electroneutral uptake of lactate, with a stoichiometry of 2 Na(+) for each lactate (By similarity).
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總結(jié) |
Normal blood lactate is maintained at about 1.5 mM, andlittle filtered lactate is excreted in urine. Reabsorption oflactate is mediated by the low-affinity Na(+)-coupled lactatetransporter SLC5A12 in the initial part of the proximal tubule andby the high-affinity Na(+)-coupled lactate transporter SLC5A8 (MIM608044) in the distal proximal tubule (Gopal et al., 2007 [PubMed17692818]).
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:2000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
Sodium-coupled monocarboxylate transporter 2, Electroneutral sodium monocarboxylate cotransporter, Low-affinity sodium-lactate cotransporter, Solute carrier family 5 member 12, SLC5A12, SMCT2
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圖像 |
Anti-SLC5A12 Antibody (C-term) at 1:2000 dilution + Y79 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST. SLC5A12 antibody (C-term) (Cat. APR10131G) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SLC5A12 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. SLC5A12 Antibody (C-term) (Cat. APR10131G) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說(shuō)明書 |
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數(shù)量 |
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