品牌 |
Leading Biology | 貨號(hào) |
APR14078G |
產(chǎn)品分類(lèi) |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality ACHE antibody (N-term).
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分子量 |
67796 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cell junction, synapse Secreted. Cell membrane; Peripheral membrane protein Isoform H: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
147-175 aa
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靶點(diǎn) |
This ACHE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-175 amino acids from the N-terminal region of human ACHE.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.
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總結(jié) |
Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. The Protein is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, IF, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
IF~~1:10~50
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別名 |
Acetylcholinesterase, AChE, ACHE
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圖像 |
ACHE Antibody (N-term) (Cat. APR14078G) western blot analysis in Jurkat,Raji,Y79 cell line lysates (35ug/lane).This demonstrates the ACHE antibody detected the ACHE protein (arrow). Formalin-fixed and paraffin-embedded human brain tissue reacted with ACHE antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. ACHE Antibody (N-term) (Cat. APR14078G) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說(shuō)明書(shū) |
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數(shù)量 |
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