品牌 |
Leading Biology | 貨號 |
APR10894G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality NDUFS4 antibody (C-term).
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分子量 |
20108 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Mitochondrion inner membrane; Peripheral membrane protein; Matrix side
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
131-160 aa
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靶點 |
This NDUFS4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-160 amino acids from the C-terminal region of human NDUFS4.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
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總結(jié) |
NDUFS4 is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase(Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. In well-coupled mitochondria, the electron flux leads to ATP generation via the building of a proton gradient across the inner membrane.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, FC, IHC-P, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial, Complex I-18 kDa, CI-18 kDa, Complex I-AQDQ, CI-AQDQ, NADH-ubiquinone oxidoreductase 18 kDa subunit, NDUFS4
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圖像 |
Western blot analysis of NDUFS4 Antibody (C-term) (Cat. APR10894G) in HL-60 cell line lysates (35ug/lane). NDUFS4 (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with NDUFS4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Flow cytometric analysis of ATDC5 cells using NDUFS4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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