品牌 |
Leading Biology | 貨號 |
APR10887G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality IL1A antibody (Center).
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分子量 |
30607 Da
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細胞定位 |
Antigen Cellular Localization:
Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins
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宿主 |
Rabbit
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種屬反應性 |
Human
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免疫原 |
177-206 aa
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靶點 |
This IL1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-206 amino acids from the Central region of human IL1A.
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亞型 |
Rabbit Ig
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通用名 |
IL1F1
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基因ID |
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UniProt ID |
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功能 |
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
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總結(jié) |
IL1A is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1, IL1A, IL1F1
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圖像 |
Western blot analysis of IL1A Antibody (Center) (Cat. APR10887G) in HepG2 cell line lysates (35ug/lane). IL1A (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human lung carcinoma reacted with IL1A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. IL1A Antibody (Center) (Cat. APR10887G) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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