CLC4 Antibody (C-term)

APR10865G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR10865G		100 μl / ￥5450

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安諾倫（北京）生物科技有限公司

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CLC4 Antibody (C-term)

品牌	Leading Biology	貨號	APR10865G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CLC4 antibody (C-term).
分子量	84917 Da
細(xì)胞定位	Antigen Cellular Localization: Early endosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	663-689 aa
靶點(diǎn)	This CLC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 663-689 amino acids from the C-terminal region of human CLC4.
亞型	Rabbit Ig
基因ID	1183
UniProt ID	P51793
功能	Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons.
總結(jié)	The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. Chloride channel 4 has an evolutionary conserved CpG island and is conserved in both mouse and hamster. This gene is mapped in close proximity to APXL (Apical protein Xenopus laevis-like) and OA1 (Ocular albinism type I), which are both located on the human X chromosome at band p22.3. The physiological role of chloride channel 4 remains unknown but may contribute to the pathogenesis of neuronal disorders.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, IF, E
稀釋方法	WB~~1:1000 IF~~1:10~50 IHC-P~~1:10~50
別名	H(+)/Cl(-) exchange transporter 4, Chloride channel protein 4, ClC-4, Chloride transporter ClC-4, CLCN4
圖像	Western blot of chicken brain tissue incubated with CLC4 Antibody (C-term) (Cat. APR10865G). Data courtesy of Emily McMains, Louisiana State University. Immunofluorescence image of cultured chick retinal amacrine (neuronal) cells labeled with CLC4 Antibody (C-term) (Cat APR10865G). Data courtesy of Emily McMains, Louisiana State University. Retinae were collected from adult White Leghorn chicken and fixed in the eyecup for two hours in 4% Paraformaldehyde (in PBS). Retinae were then removed from the eyecups and incubated in 30% sucrose (in PBS) overnight. Retinal tissue was embedded and frozen in OCT compound and cut into ~15um sections on a cryotome. Sections were blocked in 5% normal goat serum (in 1%BSA/.1%saponin PBS) for one hour and then incubated at RT with 1:250 (1%BSA/.1% saponin PBS) ClC4 antibody (Cat. APR10865G) for 1 hour. Sections were then washed in PBS (3X10minutes) and then treated with secondary antibody (1:500 Cy3) for one hour. After another PBS wash series, sections were coverslipped and antibody labeling was visualized at 20X with a Leica upright microscope using a TRITC filter set and Xenon lamp illumination. (Crousillac et al, 2003)
說明書	Product Datasheet Protocol
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