DAAM1 Antibody (N-term)

AMM08613G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
AMM08613G		100 μl / ￥5450

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安諾倫（北京）生物科技有限公司

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DAAM1 Antibody (N-term)

品牌	Leading Biology	貨號(hào)	AMM08613G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality DAAM1 antibody (N-term).
分子量	123473 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm. Note=Perinuclear
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	45-74 aa
靶點(diǎn)	This DAAM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-74 amino acids from the N-terminal region of human DAAM1.
亞型	Rabbit Ig
通用名	KIAA0666
基因ID	23002
UniProt ID	Q9Y4D1
功能	Binds to disheveled (Dvl) and Rho, and mediates Wnt- induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments.
總結(jié)	Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton. Recent evidence suggests a role for the Formin homology (FH) proteins in these processes. DAAM1 contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by DAAM1, which is thought to function as a scaffolding protein.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:10~50
別名	Disheveled-associated activator of morphogenesis 1, DAAM1, KIAA0666
圖像	Western blot analysis of DAAM1 (arrow) using rabbit polyclonal DAAM1 Antibody(Human N-term) (Cat. AMM08613G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DAAM1 gene (Lane 2) (Origene Technologies). Formalin-fixed and paraffin-embedded human brain tissue reacted with DAAM1 Antibody (N-term) (Cat. AMM08613G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
說(shuō)明書(shū)	Product Datasheet Protocol
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