品牌 |
Leading Biology | 貨號(hào) |
APR12995G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality PRPF8 antibody (C-term).
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分子量 |
273600 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Nucleus speckle.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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靶點(diǎn) |
This PRPF8 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2239-2273 amino acids from the C-terminal region of human PRPF8.
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亞型 |
Rabbit Ig
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通用名 |
PRPC8
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基因ID |
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UniProt ID |
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功能 |
Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.
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總結(jié) |
Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, E
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稀釋方法 |
WB~~1:2000
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別名 |
Pre-mRNA-processing-splicing factor 8, 220 kDa U5 snRNP-specific protein, PRP8 homolog, Splicing factor Prp8, p220, PRPF8, PRPC8
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圖像 |
Anti-PRPF8 Antibody (C-term)at 1:2000 dilution + 293T/17 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 274 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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說(shuō)明書 |
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數(shù)量 |
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