SMURF2 Antibody (N-term)

AMM08577G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢(xún)價(jià)
AMM08577G		100 μl / ￥5450

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SMURF2 Antibody (N-term)

品牌	Leading Biology	貨號(hào)	AMM08577G
產(chǎn)品分類(lèi)	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SMURF2 antibody (N-term).
分子量	86196 Da
細(xì)胞定位	Antigen Cellular Localization: Nucleus. Cytoplasm. Cell membrane. Membrane raft. Note=Cytoplasmic in the presence of SMAD7. Colocalizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse
免疫原	6-36 aa
靶點(diǎn)	This SMURF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-36 amino acids from the N-terminal region of human SMURF2.
亞型	Rabbit Ig
基因ID	64750
UniProt ID	Q9HAU4
功能	E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
總結(jié)	SMURF2, a 748-amino acid ubiquitin E3 ligase that is 83% identical to SMURF1, codes for a C2-WW-HECT domain ubiquitin ligase that associates constitutively with SMAD7. Binding to SMAD7 induces export of SMURF2 and recruitment to the activated transforming growth factor-beta receptor (TGFBR), where it causes receptor and SMAD7 degradation. A strong interaction of second and third SMURF2 WW domains has been identified with SMAD1, SMAD2, and SMAD3, but not SMAD4. Western blot analysis showed that SMURF2 selectively downregulates the transcription of SMAD2 and SMAD1, but not SMAD3. The nuclear SMURF2/phosphorylated SMAD2 interaction is requires TGFB1.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:50~100
別名	E3 ubiquitin-protein ligase SMURF2, hSMURF2, 632-, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2, SMURF2
圖像	The anti-SMURF2 Pab (Cat. AMM08577G) is used in Western blot to detect SMURF2 in mouse brain tissue lysate. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
說(shuō)明書(shū)	Product Datasheet Protocol
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