APG5 Antibody

APR07062G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR07062G		100 μl / ￥5450

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安諾倫（北京）生物科技有限公司

Tel: 400-965-8633
Tel: 010-56256916
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APG5 Antibody

品牌	Leading Biology	貨號(hào)	APR07062G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality APG5 antibody.
分子量	32447 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Note=Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme
宿主	Rabbit
種屬反應(yīng)性	Human
靶點(diǎn)	This ATG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the full length of human ATG5.
亞型	Rabbit Ig
通用名	APG5L, ASP
基因ID	9474
UniProt ID	Q9H1Y0
功能	Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12- ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.
總結(jié)	Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
形式	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by dialysis against PBS.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, IF, E
稀釋方法	WB~~1:1000 IF~~1:200 IHC-P~~1:10~50
別名	Autophagy protein 5, APG5-like, Apoptosis-specific protein, ATG5, APG5L, ASP
圖像	Western blot analysis of APG5 (arrow) using rabbit polyclonal APG5 Antibody ( AP1812c). 293T cell lysates either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5 gene. Western blot analysis of lysate from K562 cell line, using APG5 Antibody( AP1812c). AP1812c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug. Fluorescent image of U251 cells stained with ATG5 antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1812c ATG5 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
說明書	Product Datasheet Protocol
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