品牌 |
Leading Biology | 貨號(hào) |
APR00911G |
產(chǎn)品分類(lèi) |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Hdac4 Antibody - C-terminal region.
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分子量 |
118kDa
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細(xì)胞定位 |
Antigen Cellular Localization:
Nucleus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-245, Ser-465 and Ser-629 by CaMK4 and SIK1. The nuclear localization probably depends on sumoylation (By similarity).
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Mouse, Rat, Zebrafish, Horse, Yeast, Bovine, Guinea Pig, Dog
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基因ID |
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UniProt ID |
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功能 |
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D (By similarity).
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形式 |
Liquid
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, CHIP
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圖像 |
Chromatin Immunoprecipitation (ChIP) Using Hdac4 Antibody - C-terminal region and HCT116 Cells WB Suggested Anti-Hdac4 Antibody Titration: 1.0 μg/ml Positive Control: Mouse Liver Lanes: Lane 1: 40ug mouse brain, synaptosome lysate Lane 2: 40ug mouse brain, membrane fraction Lane 3: 40ug mouse brain, cytoplasm fraction Lane 4: 40ug mouse brain, nuclear fraction Lane 5: 40ug mouse brain, post synaptic density fraction Lane 6: 40ug mouse brain, synaptosome lysate Lane 7: 40ug mouse brain, membrane fraction Lane 8: 40ug mouse brain, cytoplasm fraction Lane 9: 40ug mouse brain, nuclear fraction Primary Antibody Dilution: 1:1000 Secondary Antibody: Goat anti-rabbit HRP Secondary Antibody Dilution: 1:2000 Gene Name: Hdac4 Submitted by: Wen-Cheng Xiong, Georgia Health Sciences University |
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說(shuō)明書(shū) |
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數(shù)量 |
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