PSMB7 Antibody (Center)

APR06959G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR06959G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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PSMB7 Antibody (Center)

品牌	Leading Biology	貨號	APR06959G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PSMB7 Antibody (Center).
分子量	H=30;M=30;R=30 KDa
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm {ECO:0000255\|PROSITE- ProRule:PRU00809, ECO:0000269\|PubMed:18549262}. Nucleus
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse
免疫原	180-211 aa
靶點(diǎn)	This PSMB7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 180-211 amino acids from the Central region of human PSMB7.
通用名	Z
基因ID	5695
UniProt ID	Q99436
功能	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the trypsin-like activity.
總結(jié)	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the trypsin-like activity.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB
稀釋方法	IHC~~1:25 WB~~1:8000
圖像	APR06959G staining PSMB7 in human colorectal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. All lanes : Anti-PSMB7 Antibody (Center) at 1:8000 dilution Lane 1: A375 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: MCF-7 whole cell lysate Lane 6: NIH/3T3 whole cell lysate Lane 7: Raji whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
說明書	Product Datasheet Protocol
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