品牌 |
Leading Biology |
貨號 |
APR06952G |
產品分類 |
Polyclonal Antibodies |
研究領域 |
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產品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality HDGF Antibody (C-term).
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分子量 |
H=27,26,28;M=26 KDa
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細胞定位 |
Antigen Cellular Localization:
Cytoplasm. Nucleus.
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宿主 |
Rabbit
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種屬反應性 |
Human
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免疫原 |
171-197 aa
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靶點 |
This HDGF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-197 amino acids from the C-terminal region of human HDGF.
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通用名 |
HMG1L2
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基因ID |
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UniProt ID |
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功能 |
Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor.
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總結 |
HDGF is a member of the hepatoma-derived growth factor family. This protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應用 |
IHC-P, IF, WB
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稀釋方法 |
WB~~1:2000
IHC-P~~1:10~50
IF~~1:10~50
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圖像 |
All lanes : Anti-HDGF Antibody (C-term) at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: A549 whole cell lysate
Lane 4: SK-BR-3 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 27 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HDGF antibody (C-term) (Cat.APR06952G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of HDGF Antibody (C-term)( APR06952G) with SK-BR-3 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
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說明書 |
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數量 |
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