Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CECR5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
All lanes : Anti-CECR5 Antibody (Center) at 1:1000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: HT-1080 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 46 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Flow cytometric analysis of jurkat cells using CECR5 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.