亚洲成人aⅴ综合_男人的天堂网址在线观看_日韩国产精品欧美尤物一区二区_超级性按摩中文字幕一级不长_在线观看精品国产福利片2019_中文字幕邻居人妻_最新流露出of高能预警CDwant_粉色视频高清视频版全集_产自拍视频在线_亚洲AV永久无码精品桃花岛知道

您好，歡迎光臨安諾倫（北京）生物科技商城！
全國服務(wù)熱線：400-965-8633
關(guān)注我們
  • 會員中心
  • 會員中心
    詢價(jià)列表
    0

    最新加入的商品

    共 0 件商品 合計(jì)：￥ 0
  • 會員中心
    購物車

    最新加入的商品

    共 件商品 合計(jì)：￥ 0

全部產(chǎn)品分類

自主品牌
當(dāng)前位置：首頁 > 抗原抗體、ELISA、WB > 一抗 > Polyclonal Antibodies > DKK1 Antibody

DKK1 Antibody

貨號 貨期 規(guī)格 / 價(jià)格 詢價(jià)
APR06931G 100 μl / ￥4950

DKK1 Antibody

品牌

 Leading Biology

貨號

 APR06931G

產(chǎn)品分類

 Polyclonal Antibodies

研究領(lǐng)域

產(chǎn)品概述
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality DKK1 Antibody.

分子量
H=29;M=29 KDa

細(xì)胞定位
Antigen Cellular Localization: Secreted.

宿主
Rabbit

種屬反應(yīng)性
Human, Mouse

免疫原
Recombinant protein of full sequence aa

靶點(diǎn)
This DKK1 antibody is generated from a rabbit immunized with a recombinant protein of human DKK1.

基因ID

UniProt ID

功能
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

總結(jié)
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

儲存條件
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

應(yīng)用
WB, FC, IHC-P

稀釋方法
IHC-P~~1:25 FC~~1:25 WB~~1:2000

圖像

APR06931G staining DKK1 in Human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Overlay histogram showing U-2 OS cells stained with APR06931G (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR06931G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

All lanes : Anti-DKK1 Antibody at 1:2000 dilution Lane 1: A549 whole cell lysates Lane 2: Hela whole cell lysates Lane 3: Jurkat whole cell lysates Lane 4: NIH/3T3 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

說明書

數(shù)量

相關(guān)產(chǎn)品

選擇 品牌 貨號 產(chǎn)品名稱 規(guī)格 分類 研究領(lǐng)域 說明書 數(shù)量 目錄價(jià)
1 Leading Biology APR05963G EGFR Antibody (Y1092) 100 μl Polyclonal Antibodies
￥4950.00 訂購 詢價(jià)
2 Leading Biology APR03116G BRCA1 Antibody (aa1847-1863) 100 μl Polyclonal Antibodies
￥4950.00 訂購 詢價(jià)
3 Leading Biology AMM05633G Bombesin Receptor 2 (extracellular) Antibody 50 μl Polyclonal Antibodies
￥6950.00 訂購 詢價(jià)
4 Leading Biology APR10986G AP3B1 Antibody (aa2-14) 50 μg Polyclonal Antibodies
￥4950.00 訂購 詢價(jià)
5 Leading Biology APR11512G Bestrophin-1 (extracellular) Antibody 50 μl Polyclonal Antibodies
￥6950.00 訂購 詢價(jià)
6 Leading Biology APG01407G A3 Adenosine Receptor Antibody 50 μl Polyclonal Antibodies
￥6950.00 訂購 詢價(jià)

產(chǎn)品添加成功！

購物車已有 0 件產(chǎn)品

加入詢價(jià)單成功！

詢價(jià)單已有 0 件產(chǎn)品