品牌 |
Leading Biology |
貨號 |
APG00222G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Goat Anti-NRAS Antibody.
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分子量 |
21229 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Note=Shuttles between the plasma membrane and the Golgi apparatus
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宿主 |
Goat
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種屬反應(yīng)性 |
Human, Rat
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亞型 |
IgG
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通用名 |
HRAS1
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基因ID |
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UniProt ID |
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功能 |
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
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總結(jié) |
This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML).
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形式 |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC, E
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圖像 |
HEK293 overexpressing NRAS (RC202681) and probed with APG00222G (mock transfection in first lane), tested by Origene.
APG00222G (2.5 μg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
APG00222G (0.01 μg/ml) staining of A431 lysate (35 μg protein in RIPA buffer).Primary incubation was 1 hour.Detected by chemiluminescence.
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說明書 |
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數(shù)量 |
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