MOX1 (Meox1) Antibody (N-term)

APR05873G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR05873G		100 μl / ￥4950

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MOX1 (Meox1) Antibody (N-term)

品牌	Leading Biology	貨號(hào)	APR05873G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality MOX1 (Meox1) Antibody (N-term).
分子量	27997 Da
細(xì)胞定位	Antigen Cellular Localization: Nucleus {ECO:0000250\|UniProtKB:P32442}. Cytoplasm {ECO:0000250\|UniProtKB:P32442}. Note=Localizes predominantly in the nucleus. {ECO:0000250\|UniProtKB:P32442}
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	7-38 aa
靶點(diǎn)	This MOX1 (Meox1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-38 amino acids from the N-terminal region of human MOX1 (Meox1).
亞型	Rabbit Ig
通用名	MOX1
基因ID	4222
UniProt ID	P50221
功能	Mesodermal transcription factor that plays a key role in somitogenesis and is specifically required for sclerotome development. Required for maintenance of the sclerotome polarity and formation of the cranio-cervical joints (PubMed:23290072, PubMed:24073994). Binds specifically to the promoter of target genes and regulates their expression. Activates expression of NKX3-2 in the sclerotome. Activates expression of CDKN1A and CDKN2A in endothelial cells, acting as a regulator of vascular cell proliferation. While it activates CDKN1A in a DNA-dependent manner, it activates CDKN2A in a DNA-independent manner. Required for hematopoietic stem cell (HSCs) induction via its role in somitogenesis: specification of HSCs occurs via the deployment of a specific endothelial precursor population, which arises within a sub-compartment of the somite named endotome.
總結(jié)	Meox1 is a member of a subfamily of non-clustered, diverged, antennapedia-like homeobox-containing genes. This protein may play a role in the molecular signaling network regulating somite development.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IF, E
稀釋方法	WB~~1:1000 IF~~1:10~50
圖像	Anti-Meox1 (Human N-term) at 1:1000 dilution + human heart lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of anti-Meox1 Antibody (N-term) (Cat. APR05873G) in 293 cell line lysates (35ug/lane). Meox1(arrow) was detected using the purified Pab. Fluorescent confocal image of Hela cell stained with Meox1 (Human N-term)( APR05873G).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Meox1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). Meox1 immunoreactivity is localized to Nucleolus significantly.
說(shuō)明書(shū)	Product Datasheet Protocol
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