BHLH3 Antibody (N-term)

APR05674G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢(xún)價(jià)
APR05674G		100 μl / ￥4950

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BHLH3 Antibody (N-term)

品牌	Leading Biology	貨號(hào)	APR05674G
產(chǎn)品分類(lèi)	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality BHLH3 Antibody (N-term).
分子量	50498 Da
細(xì)胞定位	Antigen Cellular Localization: Nucleus {ECO:0000255\|PROSITE- ProRule:PRU00380, ECO:0000255\|PROSITE-ProRule:PRU00981}
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	17-46 aa
靶點(diǎn)	This BHLH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human BHLH3.
亞型	Rabbit Ig
通用名	BHLHB3, DEC2, SHARP1
基因ID	79365
UniProt ID	Q9C0J9
功能	Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity.
總結(jié)	BHLHE41 may be a transcriptional repressor that represses both basal and activated transcription.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, FC, IF, E
稀釋方法	WB~~1:1000 FC~~1:10~50 IF~~1:10~50
圖像	Anti-BHLH3 Antibody (N-term) at 1:1000 dilution + human brain lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST. BHLH3 Antibody (N-term) (Cat. APR05674G) western blot analysis in RD cell line lysates (35ug/lane).This demonstrates the BHE41 antibody detected the BHE41 protein (arrow). BHLH3 Antibody (N-term) (Cat. APR05674G) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
說(shuō)明書(shū)	Product Datasheet Protocol
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