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當前位置:首頁 > 抗原抗體、ELISA、WB > 一抗 > Polyclonal Antibodies > EREG Antibody (C-term)

EREG Antibody (C-term)

貨號 貨期 規(guī)格 / 價格 詢價
APR05354G 100 μl / ¥4950

EREG Antibody (C-term)

品牌

Leading Biology

貨號

APR05354G

產品分類

Polyclonal Antibodies

研究領域

產品概述

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality EREG Antibody (C-term).

分子量

19044 Da

細胞定位

Antigen Cellular Localization: Epiregulin: Secreted, extracellular space.

宿主

Rabbit

種屬反應性

Human

免疫原

137-165 aa

靶點

This EREG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-165 amino acids from the C-terminal region of human EREG.

亞型

Rabbit Ig

基因ID

UniProt ID

功能

Ligand of the EGF receptor/EGFR and ERBB4. May be a mediator of localized cell proliferation. As a mitogen it may stimulate cell proliferation and/or angiogenesis.

總結

EREG is a member of the epidermal growth factor family. EREG can function as a ligand of EGFR (epidermal growth factor receptor), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors.

儲存條件

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

應用

WB, IHC-P, FC, E

稀釋方法

FC~~1:25 IHC-P~~1:10~50 WB~~1:1000

圖像

Overlay histogram showing HepG2 cells stained with APR05354G (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR05354G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

APR05354G staining EREG in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

APR05354G staining EREG in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

說明書

數(shù)量

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