Phospho-CCNB1(S35) Antibody

APR05338G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR05338G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

Tel: 400-965-8633
Tel: 010-56256916
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Phospho-CCNB1(S35) Antibody

品牌	Leading Biology	貨號(hào)	APR05338G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Phospho-CCNB1(S35) Antibody.
分子量	48337 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
宿主	Rabbit
種屬反應(yīng)性	Human
靶點(diǎn)	This CCNB1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S35 of human CCNB1.
亞型	Rabbit Ig
通用名	CCNB
基因ID	891
UniProt ID	P14635
功能	Essential for the control of the cell cycle at the G2/M (mitosis) transition.
總結(jié)	The protein encoded by this gene is a regulatory proteininvolved in mitosis. The gene product complexes with p34(cdc2) toform the maturation-promoting factor (MPF). Two alternativetranscripts have been found, a constitutively expressed transcriptand a cell cycle-regulated transcript, that is expressedpredominantly during G2/M phase. The different transcripts resultfrom the use of alternate transcription initiation sites. [providedby RefSeq].
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	DB, WB, E
稀釋方法	WB~~1:1100 DB~~1:500
圖像	Western blot analysis of lysate from Hela cells(from left to right),untreated or treated with Nocodazole at 50ng/ml,using Phospho-CCNB1-S35 Antibody (Cat. APR05338G) or CCNB1-S9 Antibody.Lysate at 15ug per lane.APR05338G was diluted at 1:1100 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Dot blot analysis of CCNB1 Antibody (Phospho S35) Phospho-specific Pab (Cat. APR05338G) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
說明書	Product Datasheet Protocol
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