品牌 |
Leading Biology | 貨號 |
APR04917G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality GMCL1 Antibody (Center).
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分子量 |
58685 Da
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細胞定位 |
Antigen Cellular Localization:
Nucleus matrix.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Mouse
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靶點 |
This GMCL1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 281-315 amino acids from the Central region of human GMCL1.
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亞型 |
Rabbit Ig
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通用名 |
GCL
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基因ID |
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UniProt ID |
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功能 |
Possible function in spermatogenesis. Enhances the degradation of MDM2 and increases the amount of p53 probably by modulating the nucleocytoplasmic transport (By similarity).
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總結(jié) |
Possible function in spermatogenesis. Enhances the degradation of MDM2 and increases the amount of p53 probably by modulating the nucleocytoplasmic transport (By similarity).
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
IHC-P, WB, E
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稀釋方法 |
IHC-P~~1:25
WB~~1:2000
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圖像 |
APR04917G staining GMCL1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. All lanes : Anti-GMCL1 Antibody (Center) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: mouse testis lysate Lane 4: MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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說明書 |
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數(shù)量 |
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