PPP1R13L Antibody (N-Term)

APR04846G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR04846G		100 μl / ￥4950

大規(guī)格詢價

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品牌	Leading Biology	貨號	APR04846G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PPP1R13L Antibody (N-Term).
分子量	89091 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm. Nucleus. Note=Predominantly cytoplasmic but also nuclear
宿主	Rabbit
種屬反應(yīng)性	Human
靶點(diǎn)	This PPP1R13L antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 134-166 amino acids from human PPP1R13L.
亞型	Rabbit Ig
通用名	IASPP, NKIP1, PPP1R13BL, RAI
基因ID	10848
UniProt ID	Q8WUF5
功能	Regulator that plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. Blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. Also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis.
總結(jié)	Regulator that plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. Blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. Also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	IF, WB, E
稀釋方法	IF~~1:25 WB~~1:2000
圖像	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma cell line) cells labeling Pdx1 with APR04846G at 1/25 dilution, followed by Dylight? 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight? 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue). All lanes : Anti-PPP1R13L Antibody (N-Term) at 1:2000 dilution Lane 1: MCF-7 whole cell lysates Lane 2: Hela whole cell lysates Lane 3: NCI-H1299 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 89 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
說明書	Product Datasheet Protocol
數(shù)量

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