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(Mouse) Eras Antibody (N-term)

APR04773G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR04773G		100 μl / ￥4950

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(Mouse) Eras Antibody (N-term)

品牌	Leading Biology	貨號	APR04773G
產品分類	Polyclonal Antibodies	研究領域
產品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality (Mouse) Eras Antibody (N-term).
分子量	24321 Da
細胞定位	Antigen Cellular Localization: Cell membrane; Lipid-anchor; Cytoplasmic side
宿主	Rabbit
種屬反應性	Mouse
靶點	This Mouse Eras antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 19-52 amino acids from the N-terminal region of mouse Eras.
亞型	Rabbit Ig
基因ID	353283
UniProt ID	Q7TN89
功能	Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells.
總結	Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應用	WB, FC, E
稀釋方法	FC~~1:25 WB~~1:1000
圖像	Overlay histogram showing F9 cells stained with APR04773G (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR04773G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. Western blot analysis of lysate from mouse F9 cell line, using Eras Antibody (N-term)(APR04773G). APR04773G was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
說明書	Product Datasheet Protocol
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