RBBP5 Antibody (C-term)

APR04741G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR04741G		100 μl / ￥4950

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RBBP5 Antibody (C-term)

品牌	Leading Biology	貨號	APR04741G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality RBBP5 Antibody (C-term).
分子量	59153 Da
細胞定位	Antigen Cellular Localization: Nucleus.
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse
靶點	This RBBP5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 451-485 amino acids from the C-terminal region of human RBBP5.
亞型	Rabbit Ig
通用名	RBQ3
基因ID	5929
UniProt ID	Q15291
功能	In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid (By similarity). As part of the MLL1/MLL complex, involved in mono-, di- and trimethylation at 'Lys-4' of histone H3. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation.
總結(jié)	In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid (By similarity). As part of the MLL1/MLL complex, involved in mono-, di- and trimethylation at 'Lys-4' of histone H3. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IF, E
稀釋方法	IF~~1:25 WB~~1:1000
圖像	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-2 OS (Human osteosarcoma cell line) cells labeling RBBP5 with APR04741G at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing nuclear staining on U-2 OS cell line. Cytoplasmic actin is detected with Alexa Fluor? 555 conjugated with Phalloidin (OB16636430) at 1/100 dilution (red). Western blot analysis of lysates from MCF-7, K562 cell line and mouse spleen tissue(from left to right), using RBBP5 Antibody (C-term)(APR04741G). APR04741G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
說明書	Product Datasheet Protocol
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