(Mouse) Shh Antibody (C-term)

APR04715G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR04715G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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(Mouse) Shh Antibody (C-term)

品牌	Leading Biology	貨號	APR04715G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality (Mouse) Shh Antibody (C-term).
分子量	47773 Da
細胞定位	Antigen Cellular Localization: Sonic hedgehog protein C-product: Secreted, extracellular space. Note=The C-terminal peptide diffuses from the cell
宿主	Rabbit
種屬反應性	Mouse
靶點	This (Mouse) Shh antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 397-431 amino acids from the C-terminal region of Mouse Shh.
亞型	Rabbit Ig
通用名	Hhg1
基因ID	20423
UniProt ID	Q62226
功能	Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior- posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO.
總結(jié)	Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction (By similarity).
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應用	WB, IHC-P, E
稀釋方法	IHC-P~~1:25 WB~~1:500-1:1000
圖像	Immunohistochemical analysis of paraffin-embedded M. lung section using (Mouse) Shh Antibody (C-term)( APR04715G). APR04715G was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. Western blot analysis of lysates from mouse liver, mouse stomach, rat lung tissue lysate (from left to right), using Shh Antibody (C-term)(APR04715G). APR04715G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
說明書	Product Datasheet Protocol
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