UBE2D2 Antibody (N-term)

APR04701G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR04701G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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UBE2D2 Antibody (N-term)

品牌	Leading Biology	貨號(hào)	APR04701G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality UBE2D2 Antibody (N-term).
分子量	16735 Da
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse
免疫原	7-40 aa
靶點(diǎn)	This UBE2D2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 44-76 amino acids from the N-terminal region of human UBE2D2.
亞型	Rabbit Ig
通用名	PUBC1, UBC4, UBC5B, UBCH4, UBCH5B
基因ID	7322
UniProt ID	P62837
功能	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP- induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3.
總結(jié)	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP- induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, E
稀釋方法	WB~~1:1000
圖像	All lanes : Anti-UBE2D2 Antibody (N-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: 293 whole cell lysate Lane 5: SW480 whole cell lysate Lane 6: F9 whole cell lysate Lane 7: rat testis lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of lysates from Hela, SW480, mouse NIH/3T3 cell line and rat testis tissue lysate(from left to right), using UBE2D2 Antibody (N-term)(APR04701G). APR04701G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
說(shuō)明書	Product Datasheet Protocol
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