GADD45GIP1 Antibody (N-term)

APR04627G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR04627G		100 μl / ￥4950

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GADD45GIP1 Antibody (N-term)

品牌	Leading Biology	貨號(hào)	APR04627G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GADD45GIP1 Antibody (N-term).
分子量	25384 Da
細(xì)胞定位	Antigen Cellular Localization: Mitochondrion. Nucleus. Note=Using N- terminally tagged constructs, has been found in the nucleus (PubMed:12482659). C-terminally tagged constructs are targeted exclusively to mitochondria (PubMed:22453275). This discrepancy may be explained by masking of a potential N-terminal mitochondrial targeting signal by the tag (PubMed:22453275)
宿主	Rabbit
種屬反應(yīng)性	Human
靶點(diǎn)	This GADD45GIP1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 13-45 amino acids from the N-terminal region of human GADD45GIP1.
亞型	Rabbit Ig
通用名	MRPL59, PLINP1, PRG6
基因ID	90480
UniProt ID	Q8TAE8
功能	Acts as a negative regulator of G1 to S cell cycle phase progression by inhibiting cyclin-dependent kinases. Inhibitory effects are additive with GADD45 proteins but occurs also in the absence of GADD45 proteins. Acts as a repressor of the orphan nuclear receptor NR4A1 by inhibiting AB domain-mediated transcriptional activity. May be involved in the hormone-mediated regulation of NR4A1 transcriptional activity. May play a role in mitochondrial protein synthesis.
總結(jié)	Acts as a negative regulator of G1 to S cell cycle phase progression by inhibiting cyclin-dependent kinases. Inhibitory effects are additive with GADD45 proteins but occurs also in the absence of GADD45 proteins. Acts as a repressor of the orphan nuclear receptor NR4A1 by inhibiting AB domain-mediated transcriptional activity. May be involved in the hormone-mediated regulation of NR4A1 transcriptional activity. May play a role in mitochondrial protein synthesis.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, FC, E
稀釋方法	FC~~1:25 WB~~1:1000
圖像	Flow cytometric analysis of MCF-7 cells using GADD45GIP1 Antibody (N-term)(green, APR04627G) compared to an isotype control of rabbit IgG(blue). APR04627G was diluted at 1:25 dilution. An Alexa Fluor? 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody. Western blot analysis of lysate from 293 cell line, using GADD45GIP1 Antibody (N-term) (APR04627G). APR04627G was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
說明書	Product Datasheet Protocol
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