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當(dāng)前位置：首頁(yè) > 抗原抗體、ELISA、WB > 一抗 > Polyclonal Antibodies > PTTG2 Antibody (Center)

PTTG2 Antibody (Center)

貨號(hào) 貨期 規(guī)格 / 價(jià)格 詢價(jià)
APR04153G 100 μl / ￥4950

PTTG2 Antibody (Center)

品牌

 Leading Biology

貨號(hào)

 APR04153G

產(chǎn)品分類

 Polyclonal Antibodies

研究領(lǐng)域

產(chǎn)品概述
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PTTG2 Antibody (Center).

分子量
22302 Da

細(xì)胞定位
Antigen Cellular Localization: Cytoplasm. Nucleus

宿主
Rabbit

種屬反應(yīng)性
Human

免疫原
56-84 aa

靶點(diǎn)
This PTTG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-84 amino acids from the Central region of human PTTG2.

亞型
Rabbit Ig

基因ID

UniProt ID

儲(chǔ)存條件
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

應(yīng)用
WB, IF, E

稀釋方法
WB~~1:1000 IF~~1:10~50

圖像

Anti-PTTG2 Antibody (Center) at 1:2000 dilution + human liver lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

PTTG2 Antibody (Center) (Cat. APR04153G) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PTTG2 antibody detected the PTTG2 protein (arrow).

Fluorescent confocal image of MCF-7 cell stained with PTTG2 Antibody (Center)( APR04153G).MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PTTG2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). PTTG2 immunoreactivity is localized to Cytoplasm significantly.

說(shuō)明書

數(shù)量

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