ZP4 Antibody (C-term)

APR03731G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR03731G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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ZP4 Antibody (C-term)

品牌	Leading Biology	貨號(hào)	APR03731G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ZP4 Antibody (C-term).
分子量	59400 Da
細(xì)胞定位	Antigen Cellular Localization: Processed zona pellucida sperm-binding protein 4: Secreted, extracellular space, extracellular matrix Note=The glycoproteinaceous translucent extracellular matrix that surrounds the mammalian oocyte is called zona pellucida
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	445-474 aa
靶點(diǎn)	This ZP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-474 amino acids from the C-terminal region of human ZP4.
亞型	Rabbit Ig
通用名	ZPB
基因ID	57829
UniProt ID	Q12836
功能	The mammalian zona pellucida, which mediates species- specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP4 may act as a sperm receptor.
總結(jié)	The zona pellucida is an extracellular matrix thatsurrounds the oocyte and early embryo. It is composed primarily ofthree or four glycoproteins with various functions duringfertilization and preimplantation development. The nascent proteincontains a N-terminal signal peptide sequence, a conserved ZPdomain, a consensus furin cleavage site, and a C-terminaltransmembrane domain. It is hypothesized that furin cleavageresults in release of the mature protein from the plasma membranefor subsequent incorporation into the zona pellucida matrix.However, the requirement for furin cleavage in this process remainscontroversial based on mouse studies. Previously, this gene hasbeen referred to as ZP1 or ZPB and thought to have similarfunctions as mouse Zp1. However, a human gene with highersimilarity and chromosomal synteny to mouse Zp1 has been assignedthe symbol ZP1 and this gene has been assigned the symbol ZP4.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:10~50
圖像	Anti-ZP4 Antibody (C-term) at 1:2000 dilution + NCCIT whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST. ZP4 Antibody (C-term) (Cat. APR03731G) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ZP4 antibody detected the ZP4 protein (arrow). ZP4 Antibdy (C-term) (Cat. APR03731G)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZP4 Antibdy (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
說(shuō)明書(shū)	Product Datasheet Protocol
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